Studies on the kinetic mechanism of lysine-sensitive aspartokinase.
نویسندگان
چکیده
منابع مشابه
Kinetic and Molecular Properties of Lysine-sensitive Aspartokinase
Lysine or Mg2+ alone at high concentrations brings about partial dimerization of the lysine-sensitive aspartokinase of Escherichia coli B. The native enzyme, which has a molecular weight of 75,300 and consists of 2 subunits, responds to a combination of lysine and Mg 2+ at much lower concentrations by dimerizing to a tetrameric molecule with a molecular weight approaching 150,000. This dimeriza...
متن کاملKinetic and molecular properties of lysine-sensitive aspartokinase. Factors influencing the lysine-mediated association reaction and their relationship to the cooperativity of lysine inhibition.
Lysine or Mg2+ alone at high concentrations brings about partial dimerization of the lysine-sensitive aspartokinase of Escherichia coli B. The native enzyme, which has a molecular weight of 75,300 and consists of 2 subunits, responds to a combination of lysine and Mg 2+ at much lower concentrations by dimerizing to a tetrameric molecule with a molecular weight approaching 150,000. This dimeriza...
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The increment of lysine-sensitive aspartokinase (EC 2.7.2.4) activity during in vitro culture of carrot (Daucus carota, cv. Oogata sanzun) root tissue was explained in terms of derepression caused by an earlier decrease in the endogenous level of lysine, a possible end product repressor. Tissue content of free lysine decreased to about one-third of the initial level after 1 day of culture and n...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1977
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)63347-8